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DSMZ murine macrophage cell line j774a 1
Murine Macrophage Cell Line J774a 1, supplied by DSMZ, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c j k t c 1 d ow nloaded from  (Addgene inc)
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macrophages  (DSMZ)
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DSMZ macrophages
(A) Giant unilamellar vesicles (GUVs) composed of POPC and biotin-DOPE and filled with a hyperosmotic sucrose solution (300 mOsm), leading to taut high-tension vesicles, are opsonized with AlexaFluor647 anti-biotin and mixed with LifeAct-GFP-expressing <t>macrophages.</t> During phagocytosis, the macrophage forms a phagocytic cup, identifiable from enriched actin in a ring, that encircles the GUV. The macrophage fully engulfs the GUV within tens of seconds. (B) GUVs filled with a hypoosmotic sucrose solution (270 mOsm) are at low tension and can be ‘pushed’ by a macrophage, as shown by the position of the vesicle over time relative to the mid-plane at t=0s. (C). When low-tension GUVs are trogocytosed by a macrophage, punctate ‘bites’ can be observed within the macrophage. Scale bar is 5 µm. (D-F) Time-lapse confocal microscopy images show interactions between macrophages (Lifeact-mScarlet3, pseudo-colored in green) and antibody-opsonized lymphoma cells (pseudo-colored in magenta). D) Example of a macrophage engulfing an entire target cell. E) Example of a macrophage pushing the target cell. F) Example of a macrophages trogocytosing a fragment of the target cell. Scale bars are 10 µ m. In simulations, (G) the complete engulfment of the target vesicle when its osmotic pressure is high . (H) The target is pushed when the osmotic pressure is intermediate . A plane that is shown perpendicular to the pushing direction through the middle of the target vesicle’s initial position. (I) For a very low osmotic pressure, , the cell-like vesicle takes a bite from the target.
Macrophages, supplied by DSMZ, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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raji b lymphocytes  (DSMZ)
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(A) Giant unilamellar vesicles (GUVs) composed of POPC and biotin-DOPE and filled with a hyperosmotic sucrose solution (300 mOsm), leading to taut high-tension vesicles, are opsonized with AlexaFluor647 anti-biotin and mixed with LifeAct-GFP-expressing <t>macrophages.</t> During phagocytosis, the macrophage forms a phagocytic cup, identifiable from enriched actin in a ring, that encircles the GUV. The macrophage fully engulfs the GUV within tens of seconds. (B) GUVs filled with a hypoosmotic sucrose solution (270 mOsm) are at low tension and can be ‘pushed’ by a macrophage, as shown by the position of the vesicle over time relative to the mid-plane at t=0s. (C). When low-tension GUVs are trogocytosed by a macrophage, punctate ‘bites’ can be observed within the macrophage. Scale bar is 5 µm. (D-F) Time-lapse confocal microscopy images show interactions between macrophages (Lifeact-mScarlet3, pseudo-colored in green) and antibody-opsonized lymphoma cells (pseudo-colored in magenta). D) Example of a macrophage engulfing an entire target cell. E) Example of a macrophage pushing the target cell. F) Example of a macrophages trogocytosing a fragment of the target cell. Scale bars are 10 µ m. In simulations, (G) the complete engulfment of the target vesicle when its osmotic pressure is high . (H) The target is pushed when the osmotic pressure is intermediate . A plane that is shown perpendicular to the pushing direction through the middle of the target vesicle’s initial position. (I) For a very low osmotic pressure, , the cell-like vesicle takes a bite from the target.
Raji B Lymphocytes, supplied by DSMZ, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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j774a  (DSMZ)
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(A) Giant unilamellar vesicles (GUVs) composed of POPC and biotin-DOPE and filled with a hyperosmotic sucrose solution (300 mOsm), leading to taut high-tension vesicles, are opsonized with AlexaFluor647 anti-biotin and mixed with LifeAct-GFP-expressing <t>macrophages.</t> During phagocytosis, the macrophage forms a phagocytic cup, identifiable from enriched actin in a ring, that encircles the GUV. The macrophage fully engulfs the GUV within tens of seconds. (B) GUVs filled with a hypoosmotic sucrose solution (270 mOsm) are at low tension and can be ‘pushed’ by a macrophage, as shown by the position of the vesicle over time relative to the mid-plane at t=0s. (C). When low-tension GUVs are trogocytosed by a macrophage, punctate ‘bites’ can be observed within the macrophage. Scale bar is 5 µm. (D-F) Time-lapse confocal microscopy images show interactions between macrophages (Lifeact-mScarlet3, pseudo-colored in green) and antibody-opsonized lymphoma cells (pseudo-colored in magenta). D) Example of a macrophage engulfing an entire target cell. E) Example of a macrophage pushing the target cell. F) Example of a macrophages trogocytosing a fragment of the target cell. Scale bars are 10 µ m. In simulations, (G) the complete engulfment of the target vesicle when its osmotic pressure is high . (H) The target is pushed when the osmotic pressure is intermediate . A plane that is shown perpendicular to the pushing direction through the middle of the target vesicle’s initial position. (I) For a very low osmotic pressure, , the cell-like vesicle takes a bite from the target.
J774a, supplied by DSMZ, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibodydependent cellular phagocytosis j774a 1 macrophages  (DSMZ)
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DSMZ antibodydependent cellular phagocytosis j774a 1 macrophages
(A) Giant unilamellar vesicles (GUVs) composed of POPC and biotin-DOPE and filled with a hyperosmotic sucrose solution (300 mOsm), leading to taut high-tension vesicles, are opsonized with AlexaFluor647 anti-biotin and mixed with LifeAct-GFP-expressing <t>macrophages.</t> During phagocytosis, the macrophage forms a phagocytic cup, identifiable from enriched actin in a ring, that encircles the GUV. The macrophage fully engulfs the GUV within tens of seconds. (B) GUVs filled with a hypoosmotic sucrose solution (270 mOsm) are at low tension and can be ‘pushed’ by a macrophage, as shown by the position of the vesicle over time relative to the mid-plane at t=0s. (C). When low-tension GUVs are trogocytosed by a macrophage, punctate ‘bites’ can be observed within the macrophage. Scale bar is 5 µm. (D-F) Time-lapse confocal microscopy images show interactions between macrophages (Lifeact-mScarlet3, pseudo-colored in green) and antibody-opsonized lymphoma cells (pseudo-colored in magenta). D) Example of a macrophage engulfing an entire target cell. E) Example of a macrophage pushing the target cell. F) Example of a macrophages trogocytosing a fragment of the target cell. Scale bars are 10 µ m. In simulations, (G) the complete engulfment of the target vesicle when its osmotic pressure is high . (H) The target is pushed when the osmotic pressure is intermediate . A plane that is shown perpendicular to the pushing direction through the middle of the target vesicle’s initial position. (I) For a very low osmotic pressure, , the cell-like vesicle takes a bite from the target.
Antibodydependent Cellular Phagocytosis J774a 1 Macrophages, supplied by DSMZ, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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b6 cg tyrc 2j j mice  (Bio X Cell)
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Bio X Cell b6 cg tyrc 2j j mice
(A) Giant unilamellar vesicles (GUVs) composed of POPC and biotin-DOPE and filled with a hyperosmotic sucrose solution (300 mOsm), leading to taut high-tension vesicles, are opsonized with AlexaFluor647 anti-biotin and mixed with LifeAct-GFP-expressing <t>macrophages.</t> During phagocytosis, the macrophage forms a phagocytic cup, identifiable from enriched actin in a ring, that encircles the GUV. The macrophage fully engulfs the GUV within tens of seconds. (B) GUVs filled with a hypoosmotic sucrose solution (270 mOsm) are at low tension and can be ‘pushed’ by a macrophage, as shown by the position of the vesicle over time relative to the mid-plane at t=0s. (C). When low-tension GUVs are trogocytosed by a macrophage, punctate ‘bites’ can be observed within the macrophage. Scale bar is 5 µm. (D-F) Time-lapse confocal microscopy images show interactions between macrophages (Lifeact-mScarlet3, pseudo-colored in green) and antibody-opsonized lymphoma cells (pseudo-colored in magenta). D) Example of a macrophage engulfing an entire target cell. E) Example of a macrophage pushing the target cell. F) Example of a macrophages trogocytosing a fragment of the target cell. Scale bars are 10 µ m. In simulations, (G) the complete engulfment of the target vesicle when its osmotic pressure is high . (H) The target is pushed when the osmotic pressure is intermediate . A plane that is shown perpendicular to the pushing direction through the middle of the target vesicle’s initial position. (I) For a very low osmotic pressure, , the cell-like vesicle takes a bite from the target.
B6 Cg Tyrc 2j J Mice, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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image j 1 44p program  (Nikon)
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Nikon image j 1 44p program
(A) Giant unilamellar vesicles (GUVs) composed of POPC and biotin-DOPE and filled with a hyperosmotic sucrose solution (300 mOsm), leading to taut high-tension vesicles, are opsonized with AlexaFluor647 anti-biotin and mixed with LifeAct-GFP-expressing <t>macrophages.</t> During phagocytosis, the macrophage forms a phagocytic cup, identifiable from enriched actin in a ring, that encircles the GUV. The macrophage fully engulfs the GUV within tens of seconds. (B) GUVs filled with a hypoosmotic sucrose solution (270 mOsm) are at low tension and can be ‘pushed’ by a macrophage, as shown by the position of the vesicle over time relative to the mid-plane at t=0s. (C). When low-tension GUVs are trogocytosed by a macrophage, punctate ‘bites’ can be observed within the macrophage. Scale bar is 5 µm. (D-F) Time-lapse confocal microscopy images show interactions between macrophages (Lifeact-mScarlet3, pseudo-colored in green) and antibody-opsonized lymphoma cells (pseudo-colored in magenta). D) Example of a macrophage engulfing an entire target cell. E) Example of a macrophage pushing the target cell. F) Example of a macrophages trogocytosing a fragment of the target cell. Scale bars are 10 µ m. In simulations, (G) the complete engulfment of the target vesicle when its osmotic pressure is high . (H) The target is pushed when the osmotic pressure is intermediate . A plane that is shown perpendicular to the pushing direction through the middle of the target vesicle’s initial position. (I) For a very low osmotic pressure, , the cell-like vesicle takes a bite from the target.
Image J 1 44p Program, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Giant unilamellar vesicles (GUVs) composed of POPC and biotin-DOPE and filled with a hyperosmotic sucrose solution (300 mOsm), leading to taut high-tension vesicles, are opsonized with AlexaFluor647 anti-biotin and mixed with LifeAct-GFP-expressing macrophages. During phagocytosis, the macrophage forms a phagocytic cup, identifiable from enriched actin in a ring, that encircles the GUV. The macrophage fully engulfs the GUV within tens of seconds. (B) GUVs filled with a hypoosmotic sucrose solution (270 mOsm) are at low tension and can be ‘pushed’ by a macrophage, as shown by the position of the vesicle over time relative to the mid-plane at t=0s. (C). When low-tension GUVs are trogocytosed by a macrophage, punctate ‘bites’ can be observed within the macrophage. Scale bar is 5 µm. (D-F) Time-lapse confocal microscopy images show interactions between macrophages (Lifeact-mScarlet3, pseudo-colored in green) and antibody-opsonized lymphoma cells (pseudo-colored in magenta). D) Example of a macrophage engulfing an entire target cell. E) Example of a macrophage pushing the target cell. F) Example of a macrophages trogocytosing a fragment of the target cell. Scale bars are 10 µ m. In simulations, (G) the complete engulfment of the target vesicle when its osmotic pressure is high . (H) The target is pushed when the osmotic pressure is intermediate . A plane that is shown perpendicular to the pushing direction through the middle of the target vesicle’s initial position. (I) For a very low osmotic pressure, , the cell-like vesicle takes a bite from the target.

Journal: bioRxiv

Article Title: From biting to engulfment: curvature–actin coupling controls phagocytosis of soft, deformable targets

doi: 10.64898/2026.01.28.702248

Figure Lengend Snippet: (A) Giant unilamellar vesicles (GUVs) composed of POPC and biotin-DOPE and filled with a hyperosmotic sucrose solution (300 mOsm), leading to taut high-tension vesicles, are opsonized with AlexaFluor647 anti-biotin and mixed with LifeAct-GFP-expressing macrophages. During phagocytosis, the macrophage forms a phagocytic cup, identifiable from enriched actin in a ring, that encircles the GUV. The macrophage fully engulfs the GUV within tens of seconds. (B) GUVs filled with a hypoosmotic sucrose solution (270 mOsm) are at low tension and can be ‘pushed’ by a macrophage, as shown by the position of the vesicle over time relative to the mid-plane at t=0s. (C). When low-tension GUVs are trogocytosed by a macrophage, punctate ‘bites’ can be observed within the macrophage. Scale bar is 5 µm. (D-F) Time-lapse confocal microscopy images show interactions between macrophages (Lifeact-mScarlet3, pseudo-colored in green) and antibody-opsonized lymphoma cells (pseudo-colored in magenta). D) Example of a macrophage engulfing an entire target cell. E) Example of a macrophage pushing the target cell. F) Example of a macrophages trogocytosing a fragment of the target cell. Scale bars are 10 µ m. In simulations, (G) the complete engulfment of the target vesicle when its osmotic pressure is high . (H) The target is pushed when the osmotic pressure is intermediate . A plane that is shown perpendicular to the pushing direction through the middle of the target vesicle’s initial position. (I) For a very low osmotic pressure, , the cell-like vesicle takes a bite from the target.

Article Snippet: J774A.1 macrophages and Raji B lymphocytes were obtained from DSMZ (ACC170, ACC319).

Techniques: Expressing, Confocal Microscopy